1.0 PURPOSE
This procedure ensures the efficiency of ultra violet lamp and its ability to kill the micro organisms and its validation.
2.0 SCOPE
This procedure covers the preparation of media and the method of validating the UV lamp.
3.0 RESPONSIBILITY
Head - Quality Control
Microbiologist
Assistant
4.0 PROCEDURE
4.1 Medium Nutrient agar medium:
4.2 Composition :
Peptone 0.5gm
Yeast extract 0.15gm
Beef extract 0.15gm
Sodium Chloride 0.5gm
Agar 2.0gm
Distilled water 100ml
4.3 Suspend all the ingredients in 100ml distilled water.
4.4 Sterilize at 121degC for 30 minutes.
4.5 Pour 15ml of the medium in to sterile petriplate.
4.6 Swab the staphylococcus aureus on the medium.
4.7 Expose the inoculated plate to ultra violet lamp for 15 minutes.
4.8 Incubate the place at 37 Deg C for overnight.
5.0 INTERPRETATION OF RESULTS
Absence of growth on the medium indicate the efficiency of Ultraviolet lamp.
Conduct the test for efficiency of Ultraviolet lamp, once in a month and maintain the records
6.0 ABBREVATIONS: -Nil-
7.0 REFERENCES : -Nil-
8.0 REVISION HISTORY :
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