1.0 OBJECTIVE
To provide the procedure
for the determination of Amoxycillin capsules (500mg) by UV
Spectrophometric method
2.0 SCOPE
This procedure is
Applicable to the determination of Amoxycillin capsules (500mg) by UV
Spectrophometric method
3.0 RESPONSIBILITY
3.1 QC-Chemist
4.0 ACCOUNTABILITY
4.1 Executive – Quality
control
4.2 Head – Quality
control
5.0 PROCEDURE
5.1 Label Claim:
Each Hard gelatin capsule Contains:
Amoxycillin Trihydrate IP
Equivalent to Amoxycillin ……………….500mg
5.2 Assay Method:
5.2.1 Preparation of
standard solution:
Dissolve 100mg of amoxycillin trihydrate deionized water, with using ultra sonic devise for dissolving the compound and diluting to 100mL in a volumetric flask. Futher dilute 1 ml of the above to 100 ml with deionized water.
5.2.2 Preparation of
Sample solution:
Weight powder twenty capsules
and take the powder equivalent to 100mg was
accurately weighed, dissolved in 25ml of deionized water, filtered through
Whatmann filter paper No: 41 further diluted to 100ml with deionized water,.
5.2.3 Preparation of Final Standard Solution
In a 25 mL volumetric flask, 1ml of standard solution, add 0.5 mL HCl
(0.01 N), 1.0ml of Sodium Nitrate(0.2%), and 1.0 mL of sulphanilic acid (0.5%),
1.0 mL of sodium carbonate solution(0.5 N), then make up the volume with
deionized water.
5.2.4 Prepare the Sample
Solution as per 5.2.3
5.2.5 Measure the absorbance at about 455nm
5.2.6 Calculate the content by comparison.
Sample absorbance x Standard weight x
Assay x 1 x Average weight of the capsule =
Standard absorbance x Sample Weight
x 100 x1.148
= ____________x100
LC
LC: Label Claim
6.0 ANNEXURES
Nil
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