1.0 OBJECTIVE
To provide the procedure
for the determination of Flucloxacillin
capsules (250/500mg)
2.0 SCOPE
This procedure is
Applicable to the determination of Flucloxacillin
capsules (250/500mg)
3.0 RESPONSIBILITY
3.1 QC-Chemist
4.0 ACCOUNTABILITY
4.1 Executive – Quality
control
4.2 Head – Quality
control
5.0 PROCEDURE
5.1 Label Claim:
Each capsule Contains:
Flucloxacillin IP ……………….250/500mg
5.2 Assay Method:
5.2.1 Preparation of
standard solution:
Dissolve 100mg of the Flucloxacillin in 50ml of double
distilled water and further diluted to 100ml with double distilled water. Further
dilute 1ml of the above solution into 100ml with double distilled water.
5.2.2 Preparation of
Sample solution:
Weight powder twenty
tablets and take the powder equivalent to 100mg double distilled water was
accurately weighed, dissolved in 50 ml of double distilled water, filtered
through Whatmann filter paper No: 41 further diluted to 100ml with double
distilled water.
Further dilute 1ml of
the above solution into 100ml with double distilled water.
5.2.3 Measure the absorbance at about 274nm
5.2.4 Calculate the content by comparison.
Sample absorbance x Standard weight x
Assay x Average weight of the capsule=
Standard absorbance x Sample Weight
x 100
= ____________x100
LC
LC: Label Claim
6.0 ANNEXURES
Nil
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