1.0 OBJECTIVE
To provide the procedure
for the determination of Carisoprodol tablets (350/500mg) by HPLC
method.
2.0 SCOPE
This procedure is
Applicable to the determination of Carisoprodol tablets (350/500mg) by HPLC
method.
3.0 RESPONSIBILITY
3.1 QC-Chemist
4.0 ACCOUNTABILITY
4.1 Executive – Quality
control
4.2 Head – Quality
control
5.0 PROCEDURE
5.1 Mobile Phase:
Water: acetonitrile (60:40)
5.2 Standard and sample
Preparation:
Weigh accurately about
100mg of Carisoprodol working standard in a 100ml volumetric flask and
dissolve and dilute to 100 ml with mobile Phase.
Dilute 10ml of the above
solution in to 100 ml with dilute with mobile phase upto the mark.
5.3 Sample Preparation:
Weight powder twenty
tablets and take the powder equivalent to 100mg Carisoprodol was
accurately weighed, dissolve dilute to 100ml with mobile Phase, filtered
through Whatmann filter paper No: 41.
Dilute 10ml of the above solution in to 100 ml with dilute with mobile phase upto the mark.
5.4 Chromatographic
conditions:
Mode |
Isocratic |
Flow
rate |
1.0ml
/ minute |
Injection
volume |
20µL |
Column |
C18,
250*4.6*5µm |
Run
Time |
8
minutes |
Temperature
|
300C |
Detector |
Refractive
index |
5.5
Retention Time:
Carisoprodol: 5.3 min
6.0 ANNEXURES
Nil
0 Comments