1.0 OBJECTIVE
To lay down the procedure for the Preparation and Standardization of 0.004M Benzethonium Chloride
2.0 SCOPE
This
SOP is applicable to Preparation and Standardization of 0.004M Benzethonium Chloride in company.
3.0 BACKGROUND
NIL
4.0 RESPONSIBILITY
4.1
QC-Chemist
4.2
Head-QC
5.0 PROCEDURE
5.1 Preparation:
Dissolve 1.792 g of benzethonium chloride, previously dried to constant weight at 100° to 105°, in sufficient water to produce 1000 ml.
Dissolve 1.792 g of benzethonium chloride, previously dried to constant weight at 100° to 105°, in sufficient water to produce 1000 ml.
5.2 Standardization:
Dissolve 0.35 g of the dried substance in 30 ml of anhydrous acetic acid, add 6 ml of mercury(II) acetate solution and carry out potentiometric titration with 0.1M perchloric acid using 0.5ml of crystal violet solution as an indicator to the full colour change of indicator. Carry out a blank titration.
Dissolve 0.35 g of the dried substance in 30 ml of anhydrous acetic acid, add 6 ml of mercury(II) acetate solution and carry out potentiometric titration with 0.1M perchloric acid using 0.5ml of crystal violet solution as an indicator to the full colour change of indicator. Carry out a blank titration.
Each ml of 0.1M perchloric acid VS
is equivalent to 44.81 mg of C27H42ClNO2.
5.3 Calculation
MF = W/0.04481 x V
Where
W = Weight of Benzethonium chloride in g.
V = Volume of perchloric acid consumed in ml.
MF = W/0.04481 x V
Where
W = Weight of Benzethonium chloride in g.
V = Volume of perchloric acid consumed in ml.
Note:
1) Discard the solution after 30 days.
2) Restandardise before use
3) For storage and labeling follow the general procedure
1) Discard the solution after 30 days.
2) Restandardise before use
3) For storage and labeling follow the general procedure
.JPG)
